Haemolysin production byVibrio anguillarum

Contact-haemolysin production by entero-invasive Escherichia coli and shigellae.

Entero-invasive Escherichia coli (EIEC) and shigellae were tested for contact-haemolysin (CH) with red blood cells (RBCs) of guinea-pig, rabbit, rat, mouse, monkey, man, sheep and chicken; all bacteria showed the best lysis with guinea-pig RBCs. The best culture medium for CH activity of shigellae was tryptic soy broth, and for EIEC it was casamino acid-yeast extract broth with 1 mM CaCl2. CH p...

Saline test for streptococcal haemolysin.

Salmonella typhimurium strains carrying haemolysin plasmids and cloned haemolysin genes from Escherichia coli.

Like all other Salmonella typhimurium strains examined, the smooth variants SF1397 (LT2) and 1366 and also their semi-rough and rough derivatives are non-haemolytic. Nevertheless, two haemolysin (Hly) plasmids of E. coli belonging to the inc groups incFIII,IV (pSU316) and incI2 (pHly152) were able to be introduced into these strains by conjugation and stably maintained. A considerable percentag...

Vibrio cholerae haemolysin: genetic constitution and biotype

voltage-gated K channels in other cell types [Ba 2 mM; Cs 5 mM; TEA (tetraethylammonium) 20 mM; EGTA 1 mM) (Lew & Ferreira, 1978; Egan et ul., 1987) did not, however, alter ouabainand bumetanide-insensitive K uptake. The substitution of CH,SO, for CI in the incubation medium reduced the residual K uptake by about 18%, suggesting that a Cldependent K transport pathway similar to that observed in...

Phage Therapy as an Approach to Prevent Vibrio anguillarum Infections in Fish Larvae Production

Fish larvae in aquaculture have high mortality rates due to pathogenic bacteria, especially the Vibrio species, and ineffective prophylactic strategies. Vaccination is not feasible in larvae and antibiotics have reduced efficacy against multidrug resistant bacteria. A novel approach to controlling Vibrio infections in aquaculture is needed. The potential of phage therapy to combat vibriosis in ...